ConspectusOver the past few years, the development of new materials has contributed to rapid increases in the power conversion efficiencies (PCEs) of organic photovoltaic (OPV) cells to over 17%, showing great potential for the commercialization of this technology in the near future. At this stage, designing new materials with superior performance and low cost simultaneously is of crucial importance. Chlorinated materials are emerging as new stars with very high PCEs, creating a molecular design trend to replace the most popular fluorinated materials. For example, by using chlorinated non-fullerene acceptors, we recently got a record PCE of 17% for single-junction OPV cells. Firmly based on recent advances, herein we focus on the topic of chlorinated OPV materials, aiming to provide a guideline for further molecular design.In this Account, first, on the basis of most fundamental features of the Cl atom, we highlight the features of chlorinated materials compared with their fluorinated counterparts (1) Chlorinand small-molecule donors and non-fullerene acceptors. The photovoltaic performance in various types of OPV cells using chlorinated materials, such as single-junction, tandem, semitransparent, and indoor-light photovoltaic cells is also discussed. For instance, ultranarrow-band-gap chlorinated acceptors can be used to construct highly efficient color-semitransparent OPV cells, and the wide-band-gap chlorinated materials show great potential for fabricating indoor-light photovoltaic devices. Finally, we briefly discuss current questions related to chlorinated OPV materials and highlight the significance of chlorination in future development.Although antibodies remain a primary recognition element in all forms of biosensing, functional limitations arising from their size, stability, and structure have motivated the development and production of many different artificial scaffold proteins for biological recognition. https://www.selleckchem.com/products/tak-981.html However, implementing such artificial binders into functional high-performance biosensors remains a challenging task. Here, we present the design and application of Förster resonance energy transfer (FRET) nanoprobes comprised of small artificial proteins (αRep bidomains) labeled with a Tb complex (Tb) donor on the C-terminus and a semiconductor quantum dot (QD) acceptor on the N-terminus. Specific binding of one or two protein targets to the αReps induced a conformational change that could be detected by time-resolved Tb-to-QD FRET. These single-probe FRET-switches were used in a separation-free solution-phase assay to quantify different protein targets at subnanomolar concentrations and measure the conformational changes with subnanometer resolution. Probing ligand-receptor binding under physiological conditions at very low concentrations in solution is a special feature of FRET that can be efficiently combined with other structural characterization methods to develop, understand, and optimize artificial biosensors. Our results suggest that the αRep FRET nanoprobes have a strong potential for their application in advanced diagnostics and intracellular live cell imaging of ligand-receptor interactions.Antioxidants derived from nature, such as Ellagic acid (EA), demonstrated high potency to mitigate neuronal oxidative stress and related pathologies, including Parkinson's disease (PD). However, the application of EA is limited due to its toxicity at moderate doses and poor solubility, cellular permeability, and bioavailability. Here, we introduce a sustainably resourced, green nanoencasement strategy to overcome the limitations of EA and derive synergistic effects to prevent oxidative stress in neuronal cells. Chitosan, with its high biocompatibility, potential antioxidant properties, and flexible surface chemistry, was chosen as the primary component of the nanoencasement in which EA is immobilized. Using a rotenone model to mimic intracellular oxidative stress, we examined the effectiveness of EA and chitosan to limit cell-death. Our studies indicate a synergistic effect between EA and chitosan in mitigating rotenone-induce reactive oxygen species death. Our analysis suggests that chitosan encapsulation of EA reduces the inherent cytotoxicity of the polyphenol (a known anti-cancer molecule). Furthermore, its encapsulation permits its delivery via a rapid burst phase and a relatively slow phase making the nanohybrid suitable for drug-release over extended time periods.Single-stranded DNA (ssDNA) containing four guanine repeats can form G-quadruplex (G4) structures. While cellular proteins and small molecules can bind G4s, it has been difficult to broadly assess their DNA-binding specificity. Here, we use custom DNA microarrays to examine the binding specificities of proteins, small molecules, and antibodies across ?15,000 potential G4 structures. Molecules used include fluorescently labeled pyridostatin (Cy5-PDS, a small molecule), BG4 (Cy5-BG4, a G4-specific antibody), and eight proteins (GST-tagged nucleolin, IGF2, CNBP, FANCJ, PIF1, BLM, DHX36, and WRN). Cy5-PDS and Cy5-BG4 selectively bind sequences known to form G4s, confirming their formation on the microarrays. Cy5-PDS binding decreased when G4 formation was inhibited using lithium or when ssDNA features on the microarray were made double-stranded. Similar conditions inhibited the binding of all other molecules except for CNBP and PIF1. We report that proteins have different G4-binding preferences suggesting unique cellular functions. Finally, competition experiments are used to assess the binding specificity of an unlabeled small molecule, revealing the structural features in the G4 required to achieve selectivity. These data demonstrate that the microarray platform can be used to assess the binding preferences of molecules to G4s on a broad scale, helping to understand the properties that govern molecular recognition.Molecular design strategies are crucial to develop highly efficient and long-wavelength thermally activated delayed fluorescent (TADF) emitters because the inherent limitation of the energy gap law degrades the efficiency of the red or orange TADF emitters. To resolve the low efficiency issue, we designed and synthesized two TADF emitters, 4,4'-(6-(9,9-dimethylacridin-10(9H)-yl)-7-fluoroquinoxaline-2,3-diyl)dibenzonitrile (FDQCNAc) and 11-(9,9-dimethylacridin-10(9H)-yl)-12-fluorodibenzo[a,c]phenazine-3,6-dicarbonitrile (FBPCNAc), by utilizing fluorine and peripheral cyano-substituted quinoxaline and phenazine acceptors of 4,4'-(6-fluoroquinoxaline-2,3-diyl)dibenzonitrile (FDQCN) and 11-fluorodibenzo[a,c]phenazine-3,6-dicarbonitrile (FBPCN), respectively. A fluorine atom at the ortho position of the acridine donor acts as an auxiliary acceptor to minimize the singlet-triplet energy gap (ΔEST) below 0.1 eV and promotes the reverse intersystem crossing (RISC) process. Organic light-emitting diodes (OLEDs) fabricated with FDQCNAc and FBPCNAc emitters demonstrated high external quantum efficiencies (EQEs) of 27.